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In one embodiment, the present invention is directed toward a method for identifying a gene or gene product essential for proliferation or pathogenicity of a prokaryotic organism comprising placing a nucleic acid comprising a nucleotide sequence encoding a putative essential gene under control of at least one tet operator, introducing an expression vector comprising a nucleotide sequence encoding a modified tetracycline repressor into the a prokaryotic organism, expressing the modified tetracycline repressor polypeptide, contacting the organism with a concentration of tetracycline or tetracycline analog sufficient to repress the expression level of gene product, and determining the viability of the organism. Mais j’éprouvé des malaises (maux de tête et le nez bouché)! The reaction is stined for 1 hour and subsequently dripped into cold, rapidly stined ether (500 ml). The American Society of Health-System Pharmacists. • Your urinary system is a sterile environment!
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